a:5:{s:8:"template";s:56111:" {{ keyword }}

Posted on 13/03/2023 at 3:36 am by / {{ KEYWORDBYINDEX 36 }}

{{ keyword }}About Author

{{ keyword }}Leave a reply {{ KEYWORDBYINDEX 42 }}

";s:4:"text";s:27361:"View details for Web of Science ID 000072596200032, View details for PubMedCentralID PMC19662. Bacterial cells are inherently asymmetric, some more obviously so than others. The centromere-binding protein ParB binds to and destabilizes ParA structures in vitro. In nature, this essential process occurs in cells that live in fluctuating environments. A specialized protein secretion pathway is used by some Gram-negative bacterial pathogens for delivery of virulence factors directly into mammalian host cells. Samuel Bray. Researchers from the University of British Columbia and from Professor Lucy Shapiros laboratory at Stanford also contributed to this work, which was funded in part by the National Institute of General Medical Sciences and the Chan Zuckerberg Biohub. PleC, which is required for DivJ localization, may provide the cue at the G1-to-S transition that directs the polar positioning of DivJ. View details for DOI 10.1016/j.mib.2016.06.007, View details for PubMedCentralID PMC5069156. Cell type determinants in stalked progeny promote entry into S phase, whereas swarmer progeny remain in G1 phase. The Caulobacter life cycle, defined in synchronously growing cultures, includes a sequential series of morphological changes that occur at specific times in the cycle and at specific locations in the cell. Chromosome segregation in bacteria is rapid and directed, but the mechanisms responsible for this movement are still unclear. In progeny swarmer cells, CcrM is completely degraded by Lon before its differentiation into a replication-competent stalked cell later in the cell cycle. Another regulatory mechanism involved in cell cycle progression is DNA methylation. Expression of the latter two phenotypes required complex media and both were repressed by glucose. The formation of a flagellum opposite the stalk has been observed by microscope during the differentiation of a stalked cell in preparation for cell division. The position of genetic loci on the chromosome is thereby linearly correlated with their position in the cell. Inverted-repeat nucleotide sequences in Escherichia coli and Caulobacter crescentus. Although the phospholipid composition of swarmer and stalked cells was indistinguishable in continuously labeled cultures if the two cell types were pulse-labeled for a short time period, marked differences in the pattern of phospholipid synthesis were detected. Super-resolution Imaging of Live Bacteria Cells Using a Genetically Directed, Highly Photostable Fluoromodule. The actin cytoskeleton represents a key regulator of multiple essential cellular functions in both eukaryotes and prokaryotes. Sequence comparison of the fliL transcription start site with those of other class I genes, flaS and flaO, revealed a highly conserved 29-bp sequence in a potential promoter region that differs from sigma 70, sigma 54, sigma 32, and sigma 28 promoter sequences, suggesting that at least three class I genes share a unique 5' regulatory region. Stanford Digital Economy Lab. The dimorphic bacterium Caulobacter crescentus provides a simple model for cellular differentiation. The differential turn-on of these genes contributes to the generation of asymmetry in the predivisional cell in that the products of these genes are targeted to specific cellular locations. Keiler, K. C., Shapiro, L., Williams, K. P. Identification and cell cycle control of a novel pilus system in Caulobacter crescentus, The Brucella abortus CcrM DNA methyltransferase is essential for viability, and its overexpression attenuates intracellular replication in murine macrophages. Assistant Professor, Department of Molecular and Integrative Physiology By pulsing synchronized cultures with (14)C-amino acids it has been demonstrated that the synthesis of flagellin occurs approximately 30 to 40 min before cell division. Enhanced photostability of fluorescent labels (i.e., maximum emitted photons before photobleaching) is a critical requirement for achieving the ultimate spatio-temporal resolution with either method. Nature Biotechnology (2023). Exogenous derivatives of 3':5'-cyclic GMP, 8-bromo- and N2,O2'-dibutyryl cyclic GMP, coordinately repress surface structure differentiation in Caulobacter crescentus. We combine quantitative organism-wide fluorescence imaging ("deep imaging"), functional genomics ("deep sequencing"), and statistical modeling to understand the fundamental rules that control collective cell behaviors to optimize tissue organization, regeneration, adaptation, and evolution. Carbon starvation activates DnaA proteolysis (B. Gorbatyuk and G. T. Marczynski, Mol. The level of DnaA, a key bacterial DNA replication initiation factor, increases during the Caulobacter swarmer-to-stalked transition just before the G1/S transition. Double-stranded side branches between 100 and 600 nucleotide pairs in length were visible in electron micrographs of rapidly reassociating deoxyribonucleic acid isolated by hydroxyapatite chromatography. Although transcription of flaS was not dependent on any other known gene in the flagellar hierarchy, it was autoregulated and subject to mild negative control by other genes at the same level of the hierarchy. View details for Web of Science ID A1983QR08100025. We identified a domain within the C-terminal 76 amino acids that is necessary and sufficient for accumulation as a single subcellular focus, a domain within the N-terminal 23 amino acids that is necessary for bipolar targeting, and a linker domain between these localization determinants that tolerates large variation. The DnaA regulon includes genes encoding several replisome components, the GcrA global cell cycle regulator, the PodJ polar localization protein, the FtsZ cell division protein, and nucleotide biosynthesis enzymes. In 2013, Shapiro was presented with the 2011 National Medal of Science. One of the simplest organisms to divide asymmetrically is the bacterium Caulobacter crescentus. We have isolated the dnaA gene in order to determine whether this essential and ubiquitous replication initiation protein also contributes to differential replication control in C. crescentus. Surprisingly, many signal transduction proteins are dynamically localized to specific subcellular addresses during the cell division cycle and sporulation, and proper localization is essential for their function. M.S. Their goal is to define these mechanisms using both molecular genetics and biochemistry. The Martinez lab studies RNA regulatory mechanisms that control gene expression. Despite their similar promoter architecture, these genes are transcribed at different times in the cell cycle. Home; CauloBrowser; Caulobacter Labs; Help; admin. Collaboration: In-vivo Drug Evaluation, University of Colorado and Health Sciences Center Epistatic interactions between the genes accessed by the promoter probe and other flagellar loci were studied in double fla mutants generated by transducing the promoter-probe mutations into spontaneously derived second-site fla-mutant backgrounds. Blackburn, E., Firtel, R. A., Shapiro, L. GENETIC-ANALYSIS OF A TEMPORALLY TRANSCRIBED CHEMOTAXIS GENE-CLUSTER IN CAULOBACTER-CRESCENTUS. Two lines of evidence are presented which show that an RNase III activity functions as a processing enzyme in C. crescentus. Clinical orthopaedics and related research -Coughlan, M. J., Bourdillon, A., Crisco, J. J., Kenney, D., Weiss, A., Ladd, A. L.2016: -? These genes are organized in several classes which form a transcriptional regulatory hierarchy. in Integrative Biology, University of California, Berkeley, Professor, Department of Biology, University of Utah, Adjunct Professor, Department of Human Genetics, University of Utah, Adjunct Associate Professor, Department of Human Genetics, University of Utah, Associate Professor, Department of Biology, University of Utah, Assistant Professor, Department of Biology, University of Utah, Member, Molecular Biology Program, University of Utah, Resident Biology Tutor, Leverett House, Harvard College, Research Assistant, Cardiovascular Research Center, Massachusetts General Hospital and Harvard Medical School, Research Assistant, Museum of Comparative Zoology, Harvard University, Museum Preparator, University of California Museum of Paleontology, James E. Talmage Presidential Endowed Chair, University of Utah, Myriad Genetics Award of Research Excellence, Early Career Development Award, National Science Foundation, Early Career Teaching Award, University of Utah, Career Award in the Biomedical Sciences, Burroughs Wellcome Fund, Best Symposium Presentation by a Postdoctoral Fellow, Society for Developmental Biology, Helen Hay Whitney Postdoctoral Research Fellowship, D. Dwight Davis Award for Best Student Paper, Society for Integrative and Comparative Biology, Stoye Award for Best Student Presentation, American Society of Ichthyologists and Herpetologists, Museum of Comparative Zoology/Harvard Medical School Jeffries Wyman Scholarship in Anatomy, Phi Beta Kappa, Alpha Chapter of California, BIOL 5510: Evolutionary Developmental Biology, HGEN 6091: Evolution and Development (co-taught with N. Elde, G. Kardon, and S. Sakonju), BIOL 7964: Advanced Topics in Ecology and Evolution (Team-taught course), Woods Hole MBL: Lecturer, summer Embryology course, Teaching staff, NIH Stickleback Molecular Genetics Summer Course (multiple times), Program staff, Stanford Summer Research Program, Instructor, Biology and Evolution of the Dinosauria (co-taught with L. Claessens), Teaching Fellow, Evolution of the Vertebrates (multiple times), Teaching Fellow, Structure and Physiology of the Vertebrates (multiple times), Teaching Fellow, Advanced Structure and Physiology of the Vertebrates (multiple times), Teaching Fellow, Functional and Comparative Vertebrate Anatomy (extension school), Biology tutor, Athletic Study Center and Disabled Students Program, Department of Biology, 257 South 1400 East. Here, we show that ATP depletion promotes phase separation in bacterial condensates composed of intrinsically disordered proteins. View details for Web of Science ID 000304978400010, View details for PubMedCentralID PMC3370875. Remarkably, the transcriptional circuitry is dependent on three-dimensional dynamic deployment of key regulatory and signaling proteins. We seek to understand the control of gene expression. To determine when during the cell cycle the cytoplasm is compartmentalized so that cytoplasmic proteins can no longer diffuse between the two nascent progeny cell compartments, we designed a fluorescence loss in photobleaching assay. This site provides a user-friendly interface for quickly searching genes of interest and downloading genome-wide results. Nuclease S1 analysis also revealed a protected fragment whose size was consistent with a transcript initiating in vivo at a consensus "nif" promoter sequence in front of the flaY gene. The stalked cell, which initiates chromosome replication immediately upon division, received the Lon homolog, the DnaK homolog and the 37,000 Mr RNA polymerase subunit. Mutational analysis of FliI showed that two highly conserved amino acid residues in a bipartite ATP binding motif are necessary for flagellar assembly. Despite the essential role of the CckA histidine kinase in the control of cell cycle events, the factors that signal its activation at a specific time in the cell cycle have remained elusive. We propose that the coincident transcriptional activation of several dna genes at the swarmer to stalked cell transition occurs in response to cell cycle regulatory factors, in a manner analogous to the transient transcriptional regulation of flagellar and DNA methylation genes later in the cell cycle. In wild-type cells, ATP hydrolysis opens the SMC dimer, freeing one chromosome to segregate to the opposite pole. The C. crescentus sigma32 homolog, predicted to be a 33.7-kDa protein, is 42% identical to E. coli sigma32 and cross-reacts with a monoclonal antibody to E. coli sigma32. Bacterial chromosomes are generally approximately 1000 times longer than the cells in which they reside, and concurrent replication, segregation, and transcription/translation of this crowded mass of DNA poses a challenging organizational problem. We view Developmental Biology in the broadest sense, encompassing Microbes to Humans and employing a wide variety of molecular and genetic approaches as well as systems level biology, engineering, and computational science. The methyl-accepting chemotaxis proteins (MCPs) are membrane receptors that initiate signal transduction to the flagellar rotor upon ligand binding. Because cell division then yielded a swarmer cell with a different phospholipid profile than its sibling stalked cell, the cell division process may trigger a mechanism which alters the pattern of phospholipid synthesis. Evaluation of the PROMIS Upper Extremity Against Validated Patient-Reported Outcomes in Patients with Early Carpometacarpal Osteoarthritis. Fluorescence microscopy is a sensitive tool for this purpose. for "her pioneering discovery that the bacterial cell is controlled by an integrated genetic circuit functioning in time and space that serves as a systems engineering paradigm underlying cell differentiation and ultimately the generation of diversity in all organisms." Using cluster analysis of the resulting set of 12-element vectors for each of these strains, we identified 52 strains with mutations that affected the localization pattern of the three tagged proteins. In contrast, PopZ and SpmX cannot be directly identified in CET. Research in our laboratory is focused on understanding how regulatory information encoded by the genome is integrated with the transcriptional machinery and chromatin context to allow for emergence of form and function during human embryogenesis and evolution, and how perturbations in this process lead to disease. Four metrics characterizing the observed localization patterns of each of the three labeled proteins were extracted for hundreds of cell images from each of 854 mapped mutant strains. View details for Web of Science ID A1993LX92900005, View details for Web of Science ID A1993LF06100004, View details for Web of Science ID A1993LF06100002, View details for Web of Science ID A1993KX96501075, View details for Web of Science ID A1993KX96501060. Research Assistant, Cardiovascular Research Center, Massachusetts General Hospital and Harvard Medical School. View details for Web of Science ID A1982PG49500029, View details for Web of Science ID A1981MJ92600005. Therefore phospholipid synthesis is required for stalk elongation in C. crescentus. Slowing the spread of the novel coronavirus requires testing -- lots of it. These studies provide insights into factors affecting the PleC/DivJ localization network and into regulatory links between the localization of the pili assembly protein CpaE and the kinase localization pathway. Several labeling schemes are available to accomplish this. Although ClpAP does not exhibit subcellular localization, FtsZ is a substrate of both ClpXP and ClpAPin vivo and in vitro. This sequence of proteolytic events contributes to the asymmetric localization of PodJ isoforms to the appropriate cell pole. One of these shares a promoter motif with several genes expressed at the swarmer-to-stalked cell transition; while another appears to be controlled by the CtrA global transcriptional regulator. The insertion sequence (IS) elements, IS1 and IS2, present in multiple copies in the Escherichia coli chromosome, are transposable genetic elements of known nucleotide sequence. After phage infection at least 40 proteins are phosphorylated; these include DNA-binding proteins, a membrane-associated protein, and several ribosomal proteins. American volume -Yetkinler, D. N., Ladd, A. L., Poser, R. D., Constantz, B. R., Carter, D.1999;81 (3): 391-399, JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME -Yetkinler, D. N., Ladd, A. L., Poser, R. D., Constantz, B. R., Carter, D.1999;81A (3): 391-399, JOURNAL OF HAND SURGERY-AMERICAN VOLUME -Ladd, A. L., Kibele, A., Gibbons, S.1996;21A (5): 888-897, CLINICAL ORTHOPAEDICS AND RELATED RESEARCH -Ladd, A. L., HUENE, D. S.1996: 158-171, Clinical Orthopaedics and Related ResearchLadd, A., Huene, DS1996;327: 158-171, Ladd, A. L., Kibele, A.BLACKWELL SCIENCE PUBL INC CAMBRIDGE. The histidine kinase signaling protein, PleC, that controls the temporal accumulation of the PilA pilin subunit is asymmetrically localized to the pole at which pili are assembled. We show that the peptidoglycan transpeptidase Pbp2 also forms a helical pattern that partially colocalizes with MreC but not MreB. A simple antibody-based bioassay using fluorescently tagged proteins demonstrates the encoding strategy in biologically relevant media. This origin also possesses three additional motifs that are unique to the C. crescentus origin of replication: seven 8-mer (GGCCTTCC) motifs, nine 8-mer (AAGCCCGG) motifs, and five 9-mer (GTTAA-n7-TTAA) motifs are present. A fusion of the receiver domain and last 15 residues of CtrA to YFP is properly degraded in living cells. Two genes encoding the major components of the flagellar filament, the 25K and the 27.5K flagellins, are expressed coincident with flagellar assembly. View details for Web of Science ID A1997XT77200016, View details for PubMedCentralID PMC179404. Biphasic kinetic behavior during methyl incorporation is observed when unmethylated or DNAHM substrates are used, indicating that a step after chemistry limits enzyme turnover and is most likely the release of enzyme from methylated DNA product. CHAMPER, R., Bryan, R., Gomes, S. L., Purucker, M., Shapiro, L. ANALYSIS OF THE PLEIOTROPIC REGULATION OF FLAGELLAR AND CHEMOTAXIS GENE-EXPRESSION IN CAULOBACTER-CRESCENTUS BY USING PLASMID COMPLEMENTATION. This is a sold out event but a stand by line will be made available for those who were unable to claim a ticket or be part of the wait list for the event. The CcrM protein is present only in the predivisional stage of the cell cycle, resulting in cell-cycle-dependent variation of the DNA methylation state of the chromosome. The global regulatory architecture of transcription during the Caulobacter cell cycle. Collectively, our data suggest that genome folding is globally dictated by the parS sites and chromosome segregation. WEMPireFest occurs on June 9-10, a spectacular festival celebration of the Moerner Lab work, past and present! Zhou, X., Wang, J., Herrmann, J., Moerner, W. E., Shapiro, L. Protein Self-Assembly Drives Surface Layer Biogenesis and Maintenance in C. crescentus. Bar-Zion A, Nourmahnad A, Mittelstein DR, Shivaei S, Yoo S, Buss MT, Hurt RC, Malounda D, Abedi MH, Lee-Gosselin A, Swift MB, Maresca D, Shapiro MG*. Cell wall morphogenetic protein RodA and penicillin-binding protein PBP1a also change their spatial distribution by accumulating at the division site in response to external osmotic upshifts. However, these mutants efficiently transported fatty acids and had beta-oxidation enzyme levels comparable with that of the wild type. The UBI Research Visualization bolsters basic income research by presenting existing knowledge in an accessible platform organized across multiple themes and subthemes. Small invert repeat sequences were also found flanking the individual tRNA genes. The essential elements are preferentially positioned near the origin and terminus of the chromosome. Temporally and spatially controlled master regulators drive the Caulobacter cell cycle by regulating the expression of >200 genes. We show here that one of these strains has a mutation in a homolog of the Escherichia coli secA gene, whose product is involved in protein translocation at the cell membrane. View details for DOI 10.1046/j.1365-2958.2003.03576.x, View details for Web of Science ID 000184224700005, View details for DOI 10.1073/pnas.1332806100, View details for Web of Science ID 000183845800003, View details for PubMedCentralID PMC164599. Cells use highly regulated transcriptional networks to control temporally regulated events. View details for Web of Science ID 000228496100006. Furthermore, equity in education and access is an important facet of our group's mission. Catalytic efficiency is significantly enhanced with a DNAHM substrate. The lower rings were all approximately 21 nm in diameter, although they varied significantly in width. 1997-2000. Transcription of the operon containing the structural gene for the flagellar hook protein occurs at a defined time in the cell cycle, and information necessary for transcription is contained within a region between -81 and -120 base-pairs from the transcription start site. View details for PubMedCentralID PMC3973325, View details for Web of Science ID 000346646705186, View details for Web of Science ID 000337000402130, View details for DOI 10.1016/j.bpj.2013.11.2753, View details for Web of Science ID 000337000402726, View details for DOI 10.1016/j.bpj.2013.11.408, View details for Web of Science ID 000337000400306, View details for DOI 10.1016/j.bpj.2013.11.1192, View details for Web of Science ID 000337000401138, View details for Web of Science ID 000337000401495, View details for DOI 10.1016/j.bpj.2013.11.1789, View details for Web of Science ID 000337000401688, View details for DOI 10.1016/j.bpj.2013.11.3287, View details for Web of Science ID 000337000403342, View details for DOI 10.1073/pnas.1319315110. Shapiro completed postdoctoral research at Stanford University Medical School and was named a Guggenheim Fellow at MIT's Center for Cancer Research. Our structural results also suggest that TadZ localizes to the pole through the atypical receiver domain during an early stage of pili biogenesis, and functions as a hub for recruiting other pili components, thus providing insights into the Tad pilus assembly process. With the other method, the M ring is similar to that of S. typhimurium; that is, it contacts the S ring only at an outer radius and lacks the button. The requirement for an IHF binding site and an ftr-enhancer element in spatially transcribed flagellar promoters indicates that a common mechanism may be responsible for both temporal and polar transcription. S1 protection assays and analysis of expression of the dnaK gene fused to the lux transcription reporter gene showed that expression of dnaK is temporally controlled under normal physiological conditions and that transcription occurs just before the initiation of DNA replication. Kim, S., Gitai, Z., Kinkhabwala, A., Shapiro, L., Moerner, W. E. A phospho-signaling pathway controls the localization and activity of a protease complex critical for bacterial cell cycle progression. Evidence suggests that the protein product of some fla and che genes is targeted to the incipient swarmer cell pole. Hottes, A. K., Shapiro, L., McAdams, H. H. Identification of borinic esters as inhibitors of bacterial cell growth and bacterial methyltransferases, CcrM and MenH. Complementation analysis of the Tn5 insertion mutants indicated the existence of at least four transcriptional units in the region and identified the presence of two new genes designated flbN and flbO. The mutant phenotype indicates that the assembly of the polar surface structures is coordinately regulated and independent of mechanisms regulating cell polarity and division. In addition to the motility phenotype, mutations in this locus also cause abnormal cell division. GALACTOSE CATABOLISM IN CAULOBACTER-CRESCENTUS, MEMBRANE PHOSPHOLIPID COMPOSITION OF CAULOBACTER-CRESCENTUS, SPONTANEOUS FREQUENCY OF A DEVELOPMENTAL MUTANT IN VOLVOX, CAULOBACTER-CRESCENTUS RNA-POLYMERASE - PURIFICATION AND CHARACTERIZATION OF HOLOENZYME AND CORE POLYMERASE, PH-CONDITIONAL MUTANT OF ESCHERICHIA-COLI, EFFECT OF CARBON SOURCE AND ROLE OF CYCLIC ADENOSINE-3',5'-MONOPHOSPHATE ON CAULOBACTER CELL-CYCLE, DIFFERENTIATION IN CAULOBACTER CELL-CYCLE, EFFECT OF 3'-5'-CYCLIC GMP DERIVATIVES ON FORMATION OF CAULOBACTER SURFACE-STRUCTURES, INSITU IMMUNOASSAYS FOR GENE TRANSLATION PRODUCTS IN PHAGE PLAQUES AND BACTERIAL COLONIES, CONDITIONAL SURFACE-STRUCTURE MUTANTS OF CAULOBACTER-CRESCENTUS TEMPERATURE-SENSITIVE FLAGELLA FORMATION DUE TO AN ALTERED FLAGELLIN MONOMER, STRUCTURE OF CAULOBACTER DEOXYRIBONUCLEIC-ACID, SPECIFIC CYCLIC GUANOSINE 3'-5'-MONOPHOSPHATE-BINDING PROTEIN IN CAULOBACTER-CRESCENTUS. Active segregation by a mitotic machinery appears to be common; however, the mode of chromosome segregation varies significantly from species to species. Caulobacter crescentus is a model organism for the integrated circuitry that runs a bacterial cell cycle. View details for Web of Science ID 000088132600007, View details for PubMedCentralID PMC313932. Our primary focus is on elucidating the events required for the orderly segregation of homologous chromosomes during meiosis, the crucial process by which diploid germ cells generate haploid gametes. Here we report a global transcriptional analysis of an oxygen sensory/signaling network in Caulobacter crescentus consisting of the sensor histidine kinase FixL, its cognate response regulator FixJ, the transcriptional regulator FixK, and the kinase inhibitor FixT. The cellular concentration of DnaA is cell cycle-controlled, peaking at the time of replication initiation and gcrA induction. This conclusion is based on the observations that (i) methionine auxotrophs starved of methionine can swim only in the forward direction (comparable to smooth swimming in the enteric bacteria), (ii) a specific set of membrane proteins was found to be methylated in vivo and the incorporated [3H]methyl groups were alkali sensitive, (iii) this same set of membrane proteins incorporated methyl groups from S-adenosylmethionine in vitro, and (iv) out of a total of eight generally nonchemotactic mutants, two were found to swim only in a forward direction and one of these lacked methyltransferase activity. View details for Web of Science ID A1993KT81000037, View details for Web of Science ID A1993KN46600471, View details for Web of Science ID A1993KN46600465, View details for Web of Science ID A1993KN46600478. View details for DOI 10.1016/j.tim.2005.03.006, View details for Web of Science ID 000229467100008. Ralph DiLeone, Ph.D. Graduate student, 1993-1998. Using analysis of CcrM mutant strains, transcriptional reporters integrated at different sites on the chromosome, and a ctrA P1 mutant, we demonstrate that transcription of the P1 promoter is repressed by DNA methylation. As the origin/ParB complex is being replicated and moved across the cell, PopZ accumulates at the cell pole and tethers the origin in place upon arrival. This enzyme, like RNase III isolated from Escherichia coli, processes precursor ribosomal RNAs and polycistronic phage mRNAs and has a monomeric Mr of approximately 20,000. The multiple phenotypes of the AE6000 mutant were found to cosegregate and to map between hclA and lacA on the C. crescentus chromosome. The Bejerano Lab focuses on a fundamental question in Human Genomics: the relationship between geno(me)type and phenotype. We present evidence that a bacterial signal transduction cascade that couples morphogenesis with cell cycle progression is regulated by dynamic localization of its components. ";s:7:"keyword";s:20:"shapiro lab stanford";s:5:"links";s:316:"Arxiv Submission Disappeared, Luc Schiltz Married, Articles S
";s:7:"expired";i:-1;}

{{ keyword }}Appearance > Menus

{{ keyword }}{{ keyword }}

{{ KEYWORDBYINDEX 35 }}

{{ keyword }}

{{ keyword }}About Author

{{ keyword }}

{{ keyword }}Leave a reply {{ KEYWORDBYINDEX 42 }}